Doccol 線拴是國際腦缺血研究中常用線拴，是 Nature，Stroke，J Neurosci 等雜志報(bào)道最多的線拴。根據(jù)谷歌學(xué)術(shù)的搜索結(jié)果，截止至目前，已經(jīng)有數(shù)千篇研究腦缺血的文獻(xiàn)引用了 Doccol 線栓。僅 2024 年 1 月至 12 月就有 347 篇，文獻(xiàn)目錄見我們的網(wǎng)站。美國的 Doccol 線拴是腦缺血 MCAO 模型穩(wěn)定的保證，可將腦梗塞體積標(biāo)準(zhǔn)差減少至 5%，并避免蛛網(wǎng)膜下腔出血，不需要激光多普勒檢測血流也能獲得極為穩(wěn)定的結(jié)果。同時(shí)，每只鼠 MCAO 的線栓成本可能僅為 30-50 元（可重復(fù)使用型號(hào)，視重復(fù)使用次數(shù)決定）。靶點(diǎn)科技是 Doccol 線栓中國總代理，大鼠和小鼠常用型號(hào)的栓線都是現(xiàn)貨，Doccol線栓MCAO栓線腦缺血線栓選購指南：
Doccol MCAO 線拴可以將腦梗塞體積標(biāo)準(zhǔn)差減少至 5%成功的 MCAO 模型的腦血流在插入線拴時(shí)會(huì)有斷崖式下降；在拔出線拴后，激光多普勒檢測的血流會(huì)很快恢復(fù)。采用硬質(zhì)尼龍魚線線拴，極易戳破血管，造成蛛網(wǎng)膜下腔出血（Subarachnoid hemorrhage·）。手術(shù)過程中血管如果被線拴戳破，腦血流不會(huì)有斷崖式下降，而是緩慢下降；在拔出線拴后，腦血流也不會(huì)恢復(fù)。造成腦梗塞體積變異的原因還有，不充分的梗塞血管或線拴移位造成的血流下降程度不均一及提前灌注。因此，線栓的質(zhì)地是 MCAO 模型穩(wěn)定的關(guān)鍵。
對(duì)于 MCAO 模型來說，主要有兩種線拴，無包被的火焰熔燙圓頭線拴及硅膠包被線拴。研究表明 (Tsuchiya et al. 2003)，無包被的圓頭線拴在 MCAO 實(shí)驗(yàn)過程中可能導(dǎo)致高達(dá) 40% 的蛛網(wǎng)膜下腔出血，而其標(biāo)準(zhǔn)差可能比平均值的 50% 還要大。另一項(xiàng)技術(shù)文章表明 (Shimamura et al. 2006a)，硅膠包被線拴要遠(yuǎn)好于無包被的圓頭線拴，其結(jié)果更為穩(wěn)定，即使對(duì)于沒有經(jīng)驗(yàn)的實(shí)驗(yàn)者來說也是如此。而多聚賴氨酸包被的線拴只能增加死亡率，不能減少梗賽體積的變異程度 (Huang et al. 1998)。
Doccol MCAO 線拴全部為硅膠包被，圓潤、柔軟、包被均勻，即使在沒有激光多普勒實(shí)時(shí)檢測的情況，也不會(huì)將血管戳破。使用自制的硅膠包被的線拴，大鼠 (Schmid-Elsaesser et al. 1998) 及小鼠 (Shah et al. 2006) 的腦梗塞體積的標(biāo)準(zhǔn)差均在 30% 左右。而使用 Doccol MCAO 線拴可以得到更好的結(jié)果。對(duì)于大鼠 MCAO 模型來說，使用 Doccol MCAO 線拴得到的結(jié)果的標(biāo)準(zhǔn)差可達(dá) 10%-20% （Ruscher et al.2012；Ishizaka et al.2013；Sakata et al.2012；Candelario-Jalil et al. 2008；Khan et al. 2006；Liu et al. 2006；Shimamura et al. 2006b；Solaroglu et al. 2006；Tsubokawa et al. 2007；Tsubokawa et al. 2006a；Tsubokawa et al. 2006b)。對(duì)于小鼠來說，使用 Doccol MCAO 線拴得到的結(jié)果的標(biāo)準(zhǔn)差可達(dá) 5%-10%(Chen et al.2014; Bae et al.2013; Jin et al.2011; Gu et al.2012; Kleinschnitz et al. 2007; Maysami et al. 2008; Pignataro et al. 2007b; Pignataro et al. 2007c)。
References
1. Bae ON, Serfozo K, Baek SH, et al. (2013) Safety and efficacy evaluation of carnosine, an endogenous neuroprotective agent for ischemic stroke. Stroke 44(1):205-12.
2. Candelario-Jalil E, Munoz E, Fiebich BL. (2008) Detrimental effects of tropisetron on permanent ischemic stroke in the rat. BMC Neurosci 9:19
3. Chen HZ, Guo S, Li ZZ, et al. (2014) A critical role for interferon regulatory factor 9 in cerebral ischemic stroke.J Neurosci.34(36):11897-912.
4. Huang J, Kim LJ, Poisik A, Pinsky DJ, Connolly ES, Jr. (1998) Does poly-L-lysine coating of the middle cerebral artery occlusion suture improve infarct consistency in a murine model? J Stroke Cerebrovasc Dis 7:296-301
5. Gu L, Xiong X, Zhang H, et al. (2012) Distinctive Effects of T Cell Subsets in Neuronal Injury Induced by Cocultured Splenocytes In Vitro and by In Vivo Stroke in Mice. Stroke 43:1941-1946.
6. Ishizaka S, Horie N, Satoh K, et al. (2013) Intra-arterial Cell Transplantation Provides Timing-Dependent Cell Distribution and Functional Recovery After Stroke. Stroke 44:720-726.
7. Jin R, Song Z, Yu S, et al. (2011) Phosphatidylinositol-3-kinase gamma plays a central role in blood-brain barrier dysfunction in acute experimental stroke.Stroke 42(7):2033-44.
8. Khan M, Jatana M, Elango C, Paintlia AS, Singh AK, Singh I. (2006) Cerebrovascular protection by various nitric oxide donors in rats after experimental stroke. Nitric Oxide 15:114-124
9. Kleinschnitz C, Pozgajova M, Pham M, Bendszus M, Nieswandt B, Stoll G. (2007) Targeting platelets in acute experimental stroke: impact of glycoprotein Ib, VI, and IIb/IIIa blockade on infarct size, functional outcome, and intracranial bleeding. Circulation 115:2323-2330
10. Koizumi J, Yoshida Y, Nakazawa T, Ooneda G. (1986) Experimental studies of ischemic brain edema, I: a new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke 8:1-8
11. Liu S, Liu W, Ding W, Miyake M, Rosenberg GA, Liu KJ. (2006) Electron paramagnetic resonance-guided normobaric hyperoxia treatment protects the brain by maintaining penumbral oxygenation in a rat model of transient focal cerebral ischemia. J Cereb Blood Flow Metab 26:1274-1284
12. Longa EZ, Weinstein PR, Carlson S, Cummins R. (1989) Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 20:84-91
13. Maysami S, Lan JQ, Minami M, Simon RP. (2008) Proliferating progenitor cells: a required cellular element for induction of ischemic tolerance in the brain. J Cereb Blood Flow Metab 28:1104-1113
14. Pignataro G, Simon RP, Boison D. (2007a) Transgenic overexpression of adenosine kinase aggravates cell death in ischemia. J Cereb Blood Flow Metab 27:1-5
15. Pignataro G, Simon RP, Xiong ZG. (2007b) Prolonged activation of ASIC1a and the time window for neuroprotection in cerebral ischaemia. Brain 130:151-158
16. Pignataro G, Studer FE, Wilz A, Simon RP, Boison D. (2007c) Neuroprotection in ischemic mouse brain induced by stem cell-derived brain implants. J Cereb Blood Flow Metab 27:919-927
17. Ruscher K, Kuric E, Wieloch T, et al. (2012) Levodopa Treatment Improves Functional Recovery After Experimental Stroke. Stroke 43:507-513.
18. Sakata H, et al. (2012) Minocycline-Preconditioned Neural Stem Cells Enhance Neuroprotection after Ischemic Stroke in Rats. J Neurosci. 32(10):3462–3473.
19. Schmid-Elsaesser R, Zausinger S, Hungerhuber E, Baethmann A, Reulen HJ. (1998) A critical reevaluation of the intraluminal thread model of focal cerebral ischemia: evidence of inadvertent premature reperfusion and subarachnoid hemorrhage in rats by laser-Doppler flowmetry. Stroke 29:2162-2170
20. Shah ZA, Namiranian K, Klaus J, Kibler K, Dore S. (2006) Use of an optimized transient occlusion of the middle cerebral artery protocol for the mouse stroke model. J Stroke Cerebrovasc Dis 15:133-138
21. Shimamura N, Matchett G, Tsubokawa T, Ohkuma H, Zhang J. (2006a) Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model. J Neurosci Methods 156:161-165
22. Shimamura N, Matchett G, Yatsushige H, Calvert JW, Ohkuma H, Zhang J. (2006b) Inhibition of integrin alphavbeta3 ameliorates focal cerebral ischemic damage in the rat middle cerebral artery occlusion model. Stroke 37:1902-1909
23. Solaroglu I, Tsubokawa T, Cahill J, Zhang JH. (2006) Anti-apoptotic effect of granulocyte-colony stimulating factor after focal cerebral ischemia in the rat. Neuroscience 143:965-974
24. Tsubokawa T, Jadhav V, Solaroglu I, Shiokawa Y, Konishi Y, Zhang JH. (2007) Lecithinized superoxide dismutase improves outcomes and attenuates focal cerebral ischemic injury via antiapoptotic mechanisms in rats. Stroke 38:1057-1062
25. Tsubokawa T, Solaroglu I, Yatsushige H, Cahill J, Yata K, Zhang JH. (2006a) Cathepsin and calpain inhibitor E64d attenuates matrix metalloproteinase-9 activity after focal cerebral ischemia in rats. Stroke 37:1888-1894
26. Tsubokawa T, Yamaguchi-Okada M, Calvert JW, Solaroglu I, Shimamura N, Yata K, Zhang JH. (2006b) Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats. J Neurosci Res 84:832-840
27. Tsuchiya D, Hong S, Kayama T, Panter SS, Weinstein PR. (2003) Effect of suture size and carotid clip application upon blood flow and infarct volume after permanent and temporary middle cerebral artery occlusion in mice. Brain Res 970:131-139
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